TABLE 6.

Metabolite production/incorporation in untreated and fosmidomycin-treated parasites

Metabolite	Total synthesized amount (mmol/gDW)a		$\mathbf{\Delta }\mathbf{m}\hspace{0.17em}\mathbf{=}\hspace{0.17em}\frac{{\displaystyle \int }{\mathbf{v}}_{\mathbf{FOS}}^{\mathbf{t}}}{{\displaystyle \int }{\displaystyle {\mathbf{v}}_{\mathbf{CTL}}^{\mathbf{t}}}}$
	${\displaystyle {\int }_{\mathbf{t}\mathbf{=}0}^{48}{\mathbf{v}}_{\mathbf{CTL}}^{\mathbf{t}}}$	${\displaystyle {\int }_{\mathbf{t}\mathbf{=}0}^{48}{\mathbf{v}}^{\mathbf{t}}{}_{\mathbf{FOS}}}$
Spermidine	52.8	120.2	2.28
Coenzyme A	39.4	62.2	1.58
Fe3+	32.3	46.2	1.43
Nicotinamide adenine dinucleotide oxidized	28.3	38.1	1.35
Flavin adenine dinucleotide oxidized	44.7	51.3	1.15
Putrescine	33.8	37.9	1.12
Fe2+	47.0	47.0	1.00
${\mathrm{SO}}_4^{2-}$	47.4	47.1	0.99
5,10-Methylenetetrahydrofolate	61.6	60.7	0.98
Thiamine diphosphate	46.4	44.8	0.97
S-Adenosylmethionine	348.1	318.2	0.91
10-Formyltetrahydrofolate	43.1	39.2	0.91
Riboflavin	55.3	47.0	0.85
Ubiquinol	46.1	38.5	0.83
${\mathrm{NH}}_4^{+}$	259.7	200.9	0.77
5,6,7,8-Tetrahydrofolate	75.2	46.3	0.62
Protoheme	210.6	129.3	0.61
Nicotinamide adenine dinucleotide phosphate	122.5	47.40	0.39
Pyridoxal 5-phosphate	219.9	40.69	0.19
${\mathrm{PO}}_4^{3-}$	462.0	68.72	0.15
Macromolecule	Total synthesized amount (g/gDW)		Δm
DNA	0.9	1.0	1.16
RNA	6.6	5.1	0.78
Protein	21.2	15.8	0.74
Phospholipid	2.8	1.8	0.64

^aThe total accumulated amount of a metabolite (or macromolecule) was computed by numerically integrating the synthesis rate (v) of each metabolite over the 48-h intraerythrocytic developmental cycle under control (CTL) or fosmidomycin (FOS)-treated conditions. Abbreviation: gDW, grams dry weight of the parasite.