FIG 2.

Arbidol and other approved drugs inhibit infectious PICV. Vero E6 cells were treated for 1 h with the indicated concentrations of arbidol (A), amodiaquine (B), aripiprazole (C), sertraline (D), and niclosamide (E) and infected with PICV-GFP at an MOI of 0.1. Forty-eight hours later, cells were fixed, and nuclei were counterstained with DAPI. All conditions were conducted in triplicate, and experiments were repeated 4, 1, 2, 3, and 3 times for arbidol, amodiaquine, aripiprazole, sertraline, and niclosamide, respectively. A representative curve is shown for each drug. Virus infection and cell viability were quantified by measuring GFP and DAPI fluorescence using a Cytation 1 imaging platform. For amodiaquine, cell viability was assessed by an ATPlite assay.