TABLE 2.
Plasmids
Plasmid | Description | Reference or source |
---|---|---|
pJN105 | Arabinose-inducible expression vector | 14 |
pCdrA-gfp | pUCP22Not-PcdrA-gfp(mut3)-T0-T1; Ampr Gmr | 17 |
pJBAMG10 | bifA cloned into the EcoRI-XbaI sites of pJN105 | This study |
pJN2133 | Gene PA2133 cloned into pJN105 | 15 |
pDONRminiCTX2 | Gateway-compatible version of mini-CTX2 with a 2.9-kb attP-flanking fragment from Gateway donor vector pDONR221 (Invitrogen) inserted into the HindIII site of the multiple-cloning site; Tcr Cmr | H. Almblad and J. J. Harrison |
pJBAMG13 | araC-PBAD-bifA fusion of pJBAMG10 inserted into the Gateway-compatible integration vector pDONRminiCTX2 | This study |
pENTRYminiCTX::PBAD-PA2133 | araC-PBAD-PA2133 fusion of pJN2133 inserted into the Gateway-compatible integration vector pDONRminiCTX2 | H. Almblad and J. J. Harrison |
pFlp2 | Source of Flp2 recombinase; Ampr | 39 |
pRK600 | Mobilization plasmid; Cmr | 36 |
pUX-BF13 | Tn7 transposase-encoding plasmid | 31 |
pBK-miniTn7-gfp2 | Mini-Tn7-gfp2 delivery vector | 30 |