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. 2021 Mar 18;65(4):e02431-20. doi: 10.1128/AAC.02431-20

TABLE 2.

Plasmids

Plasmid Description Reference or source
pJN105 Arabinose-inducible expression vector 14
pCdrA-gfp pUCP22Not-PcdrA-gfp(mut3)-T0-T1; Ampr Gmr 17
pJBAMG10 bifA cloned into the EcoRI-XbaI sites of pJN105 This study
pJN2133 Gene PA2133 cloned into pJN105 15
pDONRminiCTX2 Gateway-compatible version of mini-CTX2 with a 2.9-kb attP-flanking fragment from Gateway donor vector pDONR221 (Invitrogen) inserted into the HindIII site of the multiple-cloning site; Tcr Cmr H. Almblad and J. J. Harrison
pJBAMG13 araC-PBAD-bifA fusion of pJBAMG10 inserted into the Gateway-compatible integration vector pDONRminiCTX2 This study
pENTRYminiCTX::PBAD-PA2133 araC-PBAD-PA2133 fusion of pJN2133 inserted into the Gateway-compatible integration vector pDONRminiCTX2 H. Almblad and J. J. Harrison
pFlp2 Source of Flp2 recombinase; Ampr 39
pRK600 Mobilization plasmid; Cmr 36
pUX-BF13 Tn7 transposase-encoding plasmid 31
pBK-miniTn7-gfp2 Mini-Tn7-gfp2 delivery vector 30