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. 2021 May 3;220(6):e202007055. doi: 10.1083/jcb.202007055

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© 2021 Herrera et al.

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Figure 5. — β-catenin binds phosphorylated pro-N-cadherin, promoting its conversion into mature N-cadherin. (A) Western blot of whole lysates from untransfected HH16 chicken embryos probed with antibodies against pro-N-cadherin (green) and N-cadherin (red). (B) Western blot of Strep-Tactin–purified N-cadherin-ST electroporated for 24 h into HH12 chicken NTs with Sh-CTNNB1 or β-catenin and dissected at HH18. The blot was probed with antibodies against pro-N-cadherin (green), N-cadherin (red), ST (gray) and β-catenin (red). The N-cadherin/pro-N-cadherin^{upper band} ratios normalized to the transfection controls (considered 1) are shown at the bottom of each lane (n = 10). (C–E) Western blot of Strep-Tactin–purified FXa-N-cadherin-ST electroporated into HH12 chicken NTs and dissected 24 h later at HH18 and treated with EndoH (C; degrades immature N-linked glycosylation), PNGase (D; degrades all N-linked glycosylation), or AP (E; AP, dephosphorylates). The blots were probed with antibodies against pro-N-cadherin. The two pro-N-cadherin bands are labeled with blue and purple arrows. After AP treatment, the two bands turn into one; the lower one is labeled with a purple arrow. The presence of a nonspecific band is labeled as ns (n = 10). (F) Blot of Strep-Tactin–purified fractions of HH18 chicken NTs 24 hpe with N-cadherin-ST, with or without β-catenin, and treated with AP (n = 10). (G) A scheme of the proposed molecular nature of each band observed in F. (H) Same experiment as in F but with N-cadherinΔβcat (n = 10). (I) A scheme of the proposed molecular nature of each band observed in H. (J) Western blot of Strep-Tactin–purified fractions from HH18 chicken NTs 24 hpe with β-catenin·ST and probed with antibodies against pro-N-cadherin (green), N-cadherin (red), and β-catenin (green). A weaker exposure of the lysate lane is shown to the left of the main panel. Note that only the upper pro-N-cadherin band binds to β-catenin·ST (n = 15). (K) Experiment as in B but comparing the transfection of β-catenin to β-cateninΔC, a β-catenin mutant with no transcriptional activity. Numeric data are expressed as the mean ± SD (n = 10). cad, cadherin; E, elution; L, lysate; Scr, scramble; W, wash.