Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 3;220(6):e202007055. doi: 10.1083/jcb.202007055

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Herrera et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S4. — New β-catenin–dependent interactions of N-cadherin implicated in its maturation. (A) shRNAs targeting each of the seven proteins predicted to be in the Golgi apparatus were cloned into the pSHIN vector. Two different shRNAs were designed for each gene, and their knockdown efficiency was tested by quantitative RT-PCR (RT-qPCR) in chicken embryonic fibroblasts (CEFs). Each experimental condition was compared with the respective control using a one-way ANOVA with Dunnett’s multiple comparisons test (n = 3). (B) The most efficient shRNA of each pair was electroporated in HH12 chicken NTs, and slices were stained with pro-N-cadherin (red) and N-cadherin (green) at 48 hpe. GFP denoting transfection is shown in blue. Channels are shown separately in grayscale for clarity. The bar graphs show the mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001. cad, cadherin; Scr, scramble.