Figure 4.
Cryo-EM of the mitoribosome from Q/D-treated cells
(A) A model of the mitoribosomal large subunit with quinupristin and virginiamycin M1 (the hydrolysis product of dalfopristin). The two compounds are found in the entrance of the exit tunnel and the peptidyl transferase center (PTC).
(B) View of the cryo-EM density at an overall resolution of 3.9 Å around quinupristin (purple) and virginiamycin M1 (green). The two compounds interact with the surrounding rRNA.
(C) Comparison of the human mitoribosomal RNA when bound to quinupristin and virginiamycin M1 (black) and when unbound (PDB: 3J9M; light gray). Red arrows indicate rRNA movement to accommodate the molecules.