Figure 2.

miR-30a-5p negatively regulated by HDAC2 is involved in HPostC-induced autophagy inhibition. (A and B) The enrichment of H3K14 acetylation at the miR-30a-5p gene promoter was identified by chromatin immunoprecipitation-PCR analysis in cells infected with Ad-shNC and Ad-shHDAC2 for 48 h. (C) miR-30a-5p mRNA expression in senescent cardiomyocytes treated as aforementioned. (D) The relative protein expression levels of LC3B-II/I, BECN1 and p62 after knockdown of HDAC2. (E) The cell viability staining of romidepsin-treated aged H9C2 cell under normoxia, H/R or HPostC conditions (scale bar, 50 µm). Data are presented as the mean ± SD from three independent experiments. *P<0.05, **P<0.01. HPostC, hypoxia postconditioning; miR, microRNA; HDAC2, histone deacetylase 2; H/R, hypoxia/reoxygenation; Ad-, adenovirus; sh, short hairpin RNA; NC, negative control; LC3B, light chain 3β; BECN1, beclin-1.