Figure 4.

Acetylation of H3K14 promotes c-Myc binding to the miR-30a-5p gene promoter to inhibit autophagy of senescent cardiomyocytes. (A) miR-30a-5p mRNA expression was detected after knockdown of c-Myc expression under HPostC treatment. (B) Relative protein detection in the senescent cardiomyocytes treated as aforementioned. (C) Live/dead cell imaging in senescent cardiomyocytes treated as aforementioned (scale bar, 50 µm). (D) Chromatin immunoprecipitation-PCR assay of c-Myc binding at the miR-30a-5p gene promoter. (E) miR-30a-5p mRNA expression was examined by reverse transcription-quantitative PCR. Data are presented as the mean ± SD from three independent experiments. *P<0.05, **P<0.01. HPostC, hypoxia postconditioning; miR, microRNA; H/R, hypoxia/reoxygenation; Ad-, adenovirus; sh, short hairpin RNA; NC, negative control; LC3B, light chain 3β; BECN1, beclin-1.