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. 2021 Apr 20;23(6):470. doi: 10.3892/mmr.2021.12109

Figure 5.

Figure 5.

An association between circ_0058357 and miR-24-3p in NSCLC cells. (A) Relative expression level of miR-24-3p in human NSCLC tissues and paired-paracancerous tissues, as determined via RT-qPCR, (n=20/group). (B) Relative expression level of miR-24-3p in NSCLC cells, including H292, A549, H1299 and H460, and HBE cells, as detected via RT-qPCR. (C) Transfection efficiency of miR-24-3p mimics, as determined via RT-qPCR in A549 cells. (D) A549 and (E) H1299 cells were co-transfected with miR-24-3p mimic and WT or MUT-circ_0058357, then luciferase activity was detected and normalized with Renilla. (F) Transfection efficiency of miR-24-3p mimics, as determined via RT-qPCR in H1299 cells. (G) A549 and (H) H1299 cells were firstly transfected with circ_0058357 siRNA, then the relative expression of miR-24-3p was determined via RT-qPCR. **P<0.01. NC, negative control; siRNA, small interfering RNA; circ, circular RNA; HBE, human bronchial epithelial; RT-qPCR, reverse transcription-quantitative PCR; WT, wild-type; MUT, mutant; miR, microRNA.