Table 1.
Phenolic compounds | Accurate mass [M – H]− |
EE_Tamb | EE_70 | EE_100 | EE_120 | EE_MW |
---|---|---|---|---|---|---|
Phenolic acids | ||||||
Gallic acid | 169.0146 | 0.98 ± 0.06b,c | 1.07 ± 0.14b | 0.76 ± 0.04c | 0.21 ± 0.03d | 2.23 ± 0.11a |
Caffeic acid | 179.0351 | 0.18 ± 0.03b | 0.21 ± 0.04b | 0.14 ± 0.02b | 0.14 ± 0.04b | 0.35 ± 0.06a |
Flavonoids | ||||||
Myricetin 3-O-rhamnoside | 463.0891 | 0.46 ± 0.04b | 0.49 ± 0.04b | 0.37 ± 0.06b | 0.24 ± 0.03c | 0.71 ± 0.06a |
Quercetin 3-O-glucoside | 463.0955 | 0.15 ± 0.02b | 0.16 ± 0.04a,b | 0.11 ± 0.04b | 0.08 ± 0.01b | 0.24 ± 0.03a |
Quercetin 3-O-rhamnoside | 447.0769 | 0.23 ± 0.06c | 0.42 ± 0.03b | ND | ND | 0.67 ± 0.06a |
Myricetin | 317.0307 | 0.38 ± 0.07c | 0.76 ± 0.06b | 0.32 ± 0.06c | 0.27 ± 0.05c | 1.02 ± 0.02a |
Quercetin | 301.0357 | 0.42 ± 0.01b | 0.48 ± 0.06b | 0.31 ± 0.06c | 0.12 ± 0.01d | 0.77 ± 0.02a |
Kaempferol | 285.0407 | 0.12 ± 0.02a | 0.14 ± 0.05a | 0.11 ± 0.04a | 0.09 ± 0.02a | 0.16 ± 0.04a |
Total | 2.12 ± 0.13c | 2.87 ± 0.22b | 1.50 ± 0.13d | 1.08 ± 0.09d | 4.27 ± 0.30a |
HPLC profile was investigated as explained in Materials and Methods in Myrtle leaves ethanol extract subjected to different drying treatments. (EE_Tamb): leaves were dried at room temperature (16 °C); (EE_70, EE_100, and EE_120): leaves were oven dried at 70 °C, 100 °C, and 120 °C, respectively; (EE_MW): leaves were dried in microwave as reported in Materials and Method. Each value is the mean of 3 independent trials ± S.D. Data sharing different letter are significantly different at p < 0.05 (one-way ANOVA followed by Tukey Post hoc test)
ND: Not detected