Table 2.
Detection of methylation markers, cg054 and SCG3, by TAM-MSP in plasma
| ID | Diagnosis | cg054/ACTB copies | SCG3/ACTB copies | cg054 MAF (%) | SCG3 MAF (%) | Total MAF (%) |
|---|---|---|---|---|---|---|
| PCNSL_1 | PCNSL | 1/660 | 0/660 | 0.20 | 0.00 | 0.20 |
| PCNSL_2 | PCNSL | 8/1867 | 15/1867 | 0.45 | 0.80 | 1.25 |
| PCNSL_3 | PCNSL | 84/2496 | 79/2496 | 3.37 | 3.15 | 6.52 |
| PCNSL_4 | PCNSL | 9/2071 | 32/2071 | 0.42 | 1.55 | 1.97 |
| PCNSL_5 | PCNSL | 1408/2826 | 1127/2826 | 49.82 | 39.88 | 89.69 |
| PCNSL_6 | PCNSL | 0/2131 | 0/2131 | 0.00 | 0.00 | 0.00 |
| PCNSL_7 | PCNSL | 0/1536 | 0/1536 | 0.00 | 0.00 | 0.00 |
| PCNSL_8 | PCNSL | 0/243 | 0/243 | 0.00 | 0.00 | 0.00 |
| PCNSL_9 | PCNSL | 54/5158 | 0/5158 | 1.04 | 0.00 | 1.04 |
| PCNSL_10 | PCNSL | 21/2051 | 0/2051 | 1.03 | 0.00 | 1.03 |
| PCNSL_11 | PCNSL | 0/76 | 0/76 | 0.00 | 0.00 | 0.00 |
| PCNSL_12 | PCNSL | 0/7829 | 0/7829 | 0.00 | 0.00 | 0.00 |
| PCNSL_13 | PCNSL | 0/1744 | 0/1744 | 0.00 | 0.00 | 0.00 |
| PCNSL_14 | PCNSL | 2/1846 | 0/1846 | 0.09 | 0.00 | 0.09 |
| PCNSL_15 | PCNSL | 0/37 | 0/37 | 0.00 | 0.00 | 0.00 |
| Other_1 | GBM | 2/1279 | 0/1279 | 0.14 | 0.00 | 0.14 |
| Other_2 | IGa | 6/2468 | 40/2468 | 0.24 | 1.61 | 1.85 |
| Other_3 | GBM | 0/687 | 0/687 | 0.00 | 0.00 | 0.00 |
| Other_4 | GBM | 13/115 | 0/1115 | 1.18 | 0.00 | 1.18 |
| Other_5 | DMG | 0/1093 | 0/1093 | 0.00 | 0.00 | 0.00 |
| Healthy_1 | NA | 0/1756 | 4/1756 | 0.02 | 0.24 | 0.26 |
| Healthy_2 | NA | 0/2099 | 16/2099 | 0.00 | 0.76 | 0.76 |
| Healthy_3 | NA | 0/2414 | 0/2414 | 0.00 | 0.00 | 0.00 |
| Healthy_4 | NA | 0/1373 | 19/1373 | 0.00 | 1.37 | 1.37 |
| Healthy_5 | NA | 0/1529 | 0/1529 | 0.00 | 0.00 | 0.00 |
| Healthy_6 | NA | 0/1307 | 0/1307 | 0.00 | 0.00 | 0.00 |
PCNSL primary central nervous system lymphoma, MAF methylation allele frequency, other CNS tumors, GBM glioblastoma multiformae, DMG diffuse midline glioma, IG infiltrating glioma
aH3-K27M mutation