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. 2021 May 5;19:130. doi: 10.1186/s12951-021-00869-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — In vitro transfection efficiency and gene knockdown of GT/siRNA_5/10/20. a Images by confocal microscopy of HaCaT cells treated by GT/siCy5_5/10/20 complexes versus negative control (scale bars = 50 μm). b Flow cytometry analysis of transfected HaCaT cells by GT/siFAM_5/10/20 complexes. c Images by confocal microscopy of HaCaT cells incubated with LysoTracker Green and GT/siCy5₁₀ complexes for 8 h (scale bars = 20 μm). d Western Blot quantification of the cellular level of MMP-9 protein and e qRT-PCR quantification of the cellular level of MMP-9 mRNA after treatment with GT/siMMP-9_5/10/20 complexes versus negative control. *P < 0.05 compared with negative control