Table 1.
Characterization of mono-, bi-, and trifunctional nanodrugs by UV–visible spectroscopy (UV–Vis) and dynamic light scattering (DLS)
| Nanodrug | UV-Visb | DLSc | ||
|---|---|---|---|---|
| λmax (nm) | PW 75% | A650 nm/A530 nm | Radius (nm) | |
| Monofunctional | ||||
| Au/IL12 | 528 | 57 | 0.071 | 17.9 ± 2.3 |
| Au/TNF | 528 ± 2 | 58.1 ± 0.1 | 0.09 ± 0.01 | 22.1 |
| Bifunctional | ||||
| iso1Au/IL12 | 529.3 ± 0.9 | 54.5 ± 0.7 | 0.087 ± 0.02 | 21.9 ± 3.2 |
| iso1Au/TNF | 528.6 ± 0.3 | 59.0 ± 0.7 | 0.095 ± 0.01 | 21.6 ± 3.9 |
| Au/TNF + IL12a (4:1) | 531 | 52 | 0.111 | – |
| Trifunctional | ||||
| iso1Au/TNF + IL12a (1:4.5) | 529.0 ± 1.0 | 53.9 ± 0.4 | 0.113 ± 0.02 | 22.11 ± 2.6 |
| iso1Au/TNF + IL12a (4:1) | 531.9 ± 0.9 | 55.8 ± 0.3 | 0.120 ± 0.01 | – |
| Uncoated Au Nps | 524.5 ± 0.6 | 52.0 ± 4.0 | 0.065 ± 0.02 | 11.56 ± 1.4 |
aPrepared using the indicated TNF:IL12 molar ratio. See “Methods”
bλmax: wavelength of peak absorbance; PW 75%: peak width at 75% height; A650 nm/A530 nm: absorbance ratio. Mean ± SD of two different preparations
cMean ± SD of 3 measurements