Table 2.

Selected genes detected in the S. pneumoniae isolates. These parameters were used for positive gene detection: Cut-off of overlap as 80 and 95% identity. LytA (HG531769.1), ply (KP110598), Xisco (SP_1992) (AAK76059.1), rpsB (from TIGR4 AE005672.3: 2,134,264–2,135,043), pspA (AF516671), and psaA (U53509.1) were detected in all 96 isolates. cpsA (wzg) (AF057294:2134–2473), zmpB (AAK74809.1, and psrP (CS819261.1) were not detected in any of the isolates. The distribution of the genes piaA/piaB/piaC (AF338658.1), zmpC (AAK74260.1), nanA/nanB (U43526.1), and SP2020 (AAK76085.1) are presented below. The genetic antimicrobial profile of the isolates is presented below. All isolates were penicillin sensitive. The isolates were not tested for tetracycline

Reference

Year

Focus site

MLST

GPSC

PBP profile

Resistance genes

piaA

piaB

piaC

zmpC

nanA/nanB

SP2020

8–1

1950

Unknown

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

8–2

1950

Unknown

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

8–3

1962

Cerebrospinal fluid

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

8–4

1962

Cerebrospinal fluid

ST7203

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

Number of isolates

Year

Focus site

MLST

GPSC

PBP profile

Resistance genes

piaA

piaB

piaC

zmpC

nanA/nanB

SP2020

1

2006

Cerebrospinal fluid

ST53

3

3–6-5

tet(M)

Yes

Yes

Yes

Yes

Yes

Yes

1

2006

Cerebrospinal fluid

ST404

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

In 2007 was the PCV7 introduced in Denmark

1

2007

Blood

ST404

98

3–2-5

None

Yes

Yes

Yes

No

Yes

Yes

1

2007

Blood

ST404

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

5

2007

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2007

Cerebrospinal fluid

ST404

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

1

2007

Blood

ST1480

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

1

2007

Blood

ST53

3

3–6-5

tet(M)

Yes

Yes

Yes

Yes

Yes

Yes

8

2009

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2009

Cerebrospinal fluid

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2009

Blood

ST3714

224

50–0-0

None

Yes

Yes

Yes

No

Yes

Yes

In 2010 was the PCV13 introduced in Denmark

8

2010

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2010

Blood

New ST type

3

3–6-5

tet(M)

Yes

Yes

Yes

Yes

Yes

Yes

1

2010

Blood

ST1480

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

4

2011

Cerebrospinal fluid

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2011

Blood

ST53

3

3–6-5

None

Yes

No

No

Yes

Yes

Yes

5

2011

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

8

2012

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2012

Cerebrospinal fluid

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2012

Blood

ST2234

336

0–4-2

None

Yes

Yes

Yes

No

Yes

Yes

8

2013

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2013

Blood

ST53

3

3–6-5

None

No

Yes

Yes

Yes

Yes

Yes

1

2013

Blood

ST404

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

6

2014

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2014

Blood

ST53

3

3–6-5

None

Yes

No

Yes

Yes

Yes

Yes

1

2014

Blood

ST53

3

3–6-5

None

No

No

Yes

Yes

No

Yes

1

2014

Blood

ST53

3

3–6-5

None

No

No

Yes

Yes

Yes

No

1

2014

Blood

ST404

98

3–2-5

None

Yes

Yes

Yes

No

No

Yes

4

2016

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

1

2016

Blood

ST53

3

3–6-5

None

No

No

Yes

Yes

Yes

Yes

3

2016

Blood

ST53

3

3–6-5

None

Yes

No

Yes

Yes

Yes

Yes

1

2016

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

No

1

2016

Cerebrospinal fluid

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

7

2018

Blood

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes

2

2018

Blood

ST53

3

3–6-5

None

Yes

No

Yes

Yes

Yes

Yes

1

2018

Cerebrospinal fluid

ST53

3

3–6-5

None

Yes

Yes

Yes

Yes

Yes

Yes