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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Nat Genet. 2020 Sep 28;52(10):1024–1035. doi: 10.1038/s41588-020-0696-0

Figure 2. Mass spectrometry analysis identifies histone acetylation and methylation changes in aging and AD.

Figure 2.

(a) Pipeline of the proteomic experiment showing histone extraction from frozen brain tissue, histone derivatization, run of histone peptides on a nano LC-MS/MS setup, and quantification of relative histone posttranslational modifications (PTM) using EpiProfile 2.0 in Young (N = 9), Old (N = 10) and AD (N = 11). (b,c) Volcano plot showing histone PTMs changes vs. P value in the (b) Old vs. Younger and (c) AD vs. Old comparisons. The red horizontal line represents threshold of statistical significance (two-sided Student’s t-test). Significant histone acetylation changes are highlighted in red. (d-f) Barplot showing relative histone PTMs in Younger, Old and AD for histone PTMs with statistically significant differences in Old vs. Younger or in AD vs. Old. P-values are reported for statistically significant differences (P < 0.05, two-sided Student’s t-test). (g) Barplot of H3.3 vs. total H3 in Younger, Old and AD. Differences are not significant (P < 0.05, two-sided Student’s t-test). Bars in panels d-g represent the mean ± SD.