Figure 4.

SOX9 knockdown inhibits OC cells proliferation and metastasis. SOX9 siRNA was transfected into HO-8910 cells with anti-miR-185, and NC siRNA was used as control, and then cells were treated with 100 nmol/L DEX. A, cell viability detected using MTT assay; B, number of cell colonies detected using colony formation assay; C, DNA replication measured using EdU assay; D, cell apoptosis detected using flow cytometry; E, cell apoptosis rate detected using TUNEL assay; F, expression of apoptosis-related proteins detected using Western blot analysis; G, cell invasion evaluated using Transwell assay; H, cell migration detected using scratch test; all experiments were performed for 3 times and t test was used to analyze statistical significance in panels A/B/C/D/E/G/H, and two-way ANOVA and Tukey’s multiple comparisons test were used to analyze data in panel F. *, p < 0.05