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. 2021 Apr 28;77(Pt 5):134–139. doi: 10.1107/S2053230X21003952

Table 2. Conditions for crystallization of FEA1.

Method Hanging-drop vapor diffusion
Plate type VDX48 plate with sealant (Hampton Research)
Temperature (K) 277
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 40 mM Tris–HCl pH 7.5
Composition of reservoir solution 0.2 M ammonium sulfate, 0.1 M imidazole–HCl pH 6.5, 11%(w/v) PEG 3350, 30%(v/v) MPD
Volume and ratio of drop 2 µl, 1:1 ratio of protein:reservoir solution
Volume of reservoir (µl) 150