Table 1.

Crossing point (Cp), respectively cycle threshold (Ct) values by RealStar (targeting E-gene and S-gene) and Xpert (targeting E-gene and N2-gene) analysis for SARS-CoV-2 in nasopharyngeal swabs and tear fluids. COVID-19 disease severity according to WHO categorisation and timing of tear sample collection in the patient's disease course.

	Nasopharyngeal swab			Tear fluid		COVID-19 disease severity	Timing of tear sampling in disease course (days after onset of symptoms*)
	E-gene	S-gene	N2-gene	E-gene	S-gene
Patient 1	20.55	20.09	NA	–	–	Moderate	8
Patient 2	23.30	22.89	NA	–	–	Moderate	3
Patient 3	16.98	16.59	NA	–	–	Moderate	14
Patient 4	15.53	14.82	NA	34.57	33.92	Moderate	3
Patient 5	28.50	NA	31.40	–	–	Moderate	20
Patient 6	32.21	31.60	NA	–	–	Moderate	14
Patient 7	28.79	28.46	NA	–	–	Moderate	2
Patient 8	31.09	30.45	NA	–	–	Moderate	14
Patient 9	28.02	27.58	NA	–	–	Severe	5
Patient 10	21.95	21.49	NA	–	–	Moderate	2
Patient 11	32.46	31.57	NA	–	–	Severe	6
Patient 12	24.70	24.16	NA	–	–	Moderate	3
Patient 13	29.20	NA	32.00	31.36	30.86	Moderate	14
Patient 14	17.70	NA	20.20	–	–	Moderate	4
Patient 15	18.50	NA	20.90	–	–	Moderate	10
Patient 16	18.27	17.61	NA	–	–	Moderate	6
Patient 17	20.30	NA	22.50	–	–	Moderate	7
Patient 18	26.20	NA	29.10	–	–	Moderate	4
Patient 19	13.40	NA	15.50	–	–	Mild	3
Patient 20	18.70	18.12	NA	17.82	17.99	Moderate	6
Patient 21	22.36	22.02	NA	31.73	30.76	Moderate	8
Patient 22	32.98	32.27	NA	28.44	27.26	Mild	1
Patient 23	17.68	16.98	NA	28.90	28.53	Mild	2
Patient 24	26.20	NA	28.80	–	–	Moderate	6
Patient 25	16.90	16.29	NA	32.19	31.64	Moderate	4
Patient 26	32.72	31.57	NA	–	–	Moderate	14

*COVID-19 associated symptoms as defined by the WHO (eg, fever, cough, fatigue, anorexia, shortness of breath, myalgia, anosmia and ageusia).

NA, not available.