Comparative peptide sequences, biophysical properties, cellular effects and cellular permeabilities of BIM SAHBA1 and BIM SAHBA2.
| Peptide | Sequencea | α-Helicity (%) | Charge | Cellular effectsb | Cell permeabilityc | |
|---|---|---|---|---|---|---|
| Viability (μM) | Caspase 3/7 activation | |||||
| BIM SAHBA1 |
|
71 | +1 | ∼5.5 | + | + |
| BIM SAHBA2 (BimSAHB) |
|
50 | −2 | >32 | NDd | NDd |
a
Residues with negative charges are highlighted in blue; residues with positive charges are highlighted in red; X denotes residues participating in the formation of hydrocarbon staples.
b
Results on OCI-AML3 cells are shown here.
c
Cell permeability was verified by electron microscopy and immunoelectron microscopy; an endosomal mechanism of cell entry was confirmed.
d
Not determined.