Comparative α-helicity, hydrophobicity and cell permeability orders of the model peptide targeting the ER coactivatora.
| |||
|---|---|---|---|
|
Helicity order | Hydrophobicity order | Cell permeability orderb |
| Hydrocarbon | 1 | 1 | 4 |
| Lactam | 1 | 6 | 6 |
| Triazole | 3 | 5 | 5 |
| Vinyl sulfide | 4 | 4 | 3 |
| m-Xylene | 5 | 3 | 2 |
| Perfluoroaryl | 7 | 2 | 1 |
| None (linear) | 6 | 6 | 7 |
a
The same numbers denote the two stapled peptide showed similar specific properties.
b
The cell-permeabilities were detected using FAM-labelled stapled peptides in T47D, MCF-7, Hela, HEK293T cell lines via confocal microscopy, which showed concordant results in this study.