Fig. 3. Site-specific profiling of itaconated cysteines by C3A in S. enterica lysates. (A) The TOP-ABPP workflow for chemoproteomic profiling of itaconated sites in S. enterica lysates. (B) Overlap of the itaconated cysteines identified by C3A in two biological replicates. (C) Distribution of the itaconation targets in terms of the number of modification sites per protein. (D) Biochemical validation of the novel itaconation sites in ENO, ORN, YEBC, ATPG, YHBO, and YIEF by comparing the C3A labeling between the wild-type (WT) proteins and their cysteine-to-serine mutants.
