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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Prog Brain Res. 2011;194:131–144. doi: 10.1016/B978-0-444-53815-4.00011-X

Fig. 7.

Fig. 7.

In situ histology to investigate the intact device/tissue interface. (a) Transmission light image showing a microelectrode array captured in fixed brain tissue and imaged by laser confocal microscopy. (b) Microglia, labeled by immunohistochemically tagging the protein Iba1 with the fluorescent marker Alexa Fluor 633, are seen in a single optical section responding in a layer-dependent fashion around this 1-week implanted device. (c) A 10-μm-thick z-stack of images shows the device surface imaged by collecting laser reflectance; (d) microglia at this same location are shown responding to the presence of the device 24 h after implantation. (e) Microglia filopodia investigating the device’s surface and neighboring tissue are further presented in this image overlay. Scale bars indicate 200 μm in (a) and (b), and 50 μm in (c–e).