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. 2021 Feb 15;32(4):314–330. doi: 10.1091/mbc.E20-10-0622

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© 2021 Menon et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Myo16 and LPPR4 colocalize with TRIM67. (Ai) Representative images to demonstrate colocalization of GFP-Myo16 with tagRFP-TRIM67∆RING. Zoomed inset and the blue arrowheads show colocalization along the axon and in filopodia along the axon. (Aii) Pearson’s correlation of colocalization between GFP-Myo16 and tagRFP-TRIM67∆RING (R_obs) compared with Fay-randomized controls (R_rand). (n = 9 cells from three biological replicates.) (Bi) Representative images to demonstrate colocalization of GFP-LPPR4 with tagRFP-TRIM67. Zoomed inset and the blue arrowheads show colocalization along the axon (yellow box) and at the axonal growth cone (red box). (Bii) Pearson’s correlation of colocalization between GFP-LPPR4 and tagRFP-TRIM67 (R_obs) compared with Fay-randomized controls (R_rand). (n = 12 cells from four biological replicates.) Datapoints were compared using Student’s t test, ***, P < 0.005.