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. 2021 Feb 15;32(4):331–347. doi: 10.1091/mbc.E20-07-0492

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FIGURE 3: — MT dynamics and polarity in mec-7 lf, anti, and neo mutants. (A) Representative kymographs of MT dynamics in the PLM-AN and PLM-PN of adult WT, mec-7(u1122lf; S145F), and mec-7(u1110anti; L225F) animals. (B) The total number of EBP-2::GFP comets in a 60-μm segment of PLM-AN and a 25-μm segment of PLM-PN from the cell body in WT, mec-7(u1122lf), and mec-7(u1110anti) animals. The fraction of MTs with plus-end-out (anterograde direction comets) and “minus-end-out” (retrograde direction comets) polarity in PLM-AN and PLM-PN. (C) Representative kymographs of MT dynamics in WT and mec-7(u278neo; C303Y) animals, which were treated with 0.125 mM colchicine for 8 h at L4 stage and then recovered on regular NGM plates for 1 h. (D) The number of EBP-2::GFP comets and the fraction of the comets in anterograde and retrograde directions in the treated WT and mec-7(u278neo) animals.