FIGURE 6:

Structural modeling of the tubulin neo mutations. (A) Positions of the amino acids affected by mec-7 neo alleles. C303Y (yellow) is the strongest mec-7 neo alleles. Others (orange) are much weaker than C303Y mutation. (B) Positions of the amino acids affected by mec-12 neo alleles. In A and B, mutations with asterisks were isolated in this study and not analyzed before. (C) Modeling of the C303Y substitution with two possible orientations. Left panel shows a configuration with 60% possibility according to ChimeraX, which creates clashes (purple dashed lines) with L377 and E376 residues. Right panel shows another configuration with 18% possibility, leading to clashes with M267. (D) C303S substitution did not lead to any clashes with surrounding residues. (E) A302T and A302V substitutions led to clashes with D203 residue. C303 and A302 are labeled in yellow and orange, respectively.