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. 2021 Jan 1;32(1):45–56. doi: 10.1091/mbc.E20-08-0556

FIGURE 2:

FIGURE 2:

The C-terminal α-helix region of IFT88 is crucial for its interaction with the IFT-A complex. (A, B) Schematic representation of the IFT88 constructs. (A) Domain organization of IFT88(WT) and its deletion constructs. (B) Secondary structure of the C-terminal TPR and α-helix regions of the IFT88 protein, predicted by NetSurfP-1.0 (Petersen et al., 2009). (C, D) Lysates of cells coexpressing the mChe-fused IFT88 constructs, as indicated, plus IFT52 and IFT46, and EGFP-fused IFT144+IFT122 were subjected to the VIP assay (C) or immunoblotting analysis (D). (E, F) Lysates of cells coexpressing EGFP-fused IFT88 constructs, as indicated, plus IFT52 and IFT46, and all the IFT-A subunits (IFT144/IFT140/IFT139/IFT122/IFT121/IFT43) fused to mChe were subjected to the VIP assay (E) or immunoblotting analysis (F).