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. 2021 Jan 1;32(1):45–56. doi: 10.1091/mbc.E20-08-0556

FIGURE 3:

FIGURE 3:

The C-terminal α-helix region of IFT88 does not play an important role in its interactions with other IFT-B subunits and kinesin-II. (A, B) Lysates of cells coexpressing EGFP-fused IFT88 constructs, as indicated, and mChe-IFT52 were subjected to the VIP assay (A) or immunoblotting analysis (B). (C, D) Lysates of cells coexpressing EGFP-IFT70B and mChe-fused IFT88 constructs, as indicated, plus IFT52 were subjected to the VIP assay (C) or immunoblotting analysis (D). (E, F) Lysates of cells coexpressing EGFP-fused IFT88 constructs, as indicated, plus IFT52 and tRFP-fused IFT57+IFT38 were subjected to the VIP assay (E) or immunoblotting analysis (F). (G, H) Lysates of cells coexpressing EGFP-fused kinesin-II subunits and the mChe-IFT88 constructs, as indicated, plus mChe/tRFP-fused IFT57+IFT52+IFT38 were subjected to the VIP assay (G) or immunoblotting analysis (H). Note that, in the immunoblotting analyses shown in B, D, F, and H, anti-RFP and anti-tRFP antibodies recognize mChe and tRFP, respectively. (I) Summary of the results of the interaction analyses shown in Figures 2 and 3. +, firm interaction; ±, weak interaction; ND, not determined.