FIGURE 4:

Accumulation of R-loops contributes to the enrichment of ssDNA at the CEN chromatin. (A) Accumulation of R-loops at CEN coincides with the enrichment of CEN-derived ssDNA in an hpr1Δ strain. DRIP-qPCR analysis of WT (BY4741) and hpr1Δ (BY4741 hpr1Δ) strains was performed using the S9.6 (anti–DNA-RNA hybrid) antibodies. Average from three biological replicates ± SE. **p value < 0.01, Student’s t test. (B) Expression of CEN lncRNA in S-phase is largely similar between WT and hpr1Δ strains. Total RNA was used in RT-qPCR to determine the expression level of CEN lncRNA (CEN1, CEN3, CEN6, CEN7, and CEN8) and ACT1 lncRNA (control). Average from three biological replicates ± SE. ns = statistically not significant, Student’s t test.