TABLE 1:

Budding yeast strains, plasmids, and primers used in this study.

Saccharomyces cerevisiae
Strain	Genotype		Reference
BY4741	MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0		Open Biosystems
BY4741 hpr1Δ	MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 hpr1Δ::KAN		Open Biosystems
YPH1018	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1 CFIII (CEN3L.YPH278) HIS3 SUP11		Phil Hieter, University of British Columbia, Vancouver, BC, Canada
JG595	MATa ura3-1 leu2,3-112 his3-1 trp1-1 ade2-1 can1-100 Δbar1 CSE4-12Myc::URA3 SCM3-3Flag::KANMX		Camahort et al., 2007
YMB11085	MATa ura3-1 leu2,3-112 his3-1 trp1-1 ade2-1 can1-100 Δbar1 CSE4-12Myc::URA3 SCM3-3Flag::KANMX HPR1-3HA::HIS3		This study
YMB11087	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3-∆200 leu2∆1 hpr1∆::TRP1 CFIII (CEN3L.YPH278) HIS3 SUP11		This study
YMB11097	MATa ura3-1 leu2,3-112 his3-1 trp1-1 ade2-1 can1-100 Δbar1 CSE4-12Myc::URA3 SCM3-3Flag::KANMX hpr1∆::TRP1		This study
YMB11210	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1 URA3-Vector (pRS426)		This study
YMB11211	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1 pGPD-RNH1-HA::URA3 (pBB39)		This study
YMB11212	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1 hpr1∆::TRP1 URA3-Vector (pRS426)		This study
YMB11213	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1 hpr1∆::TRP1 pGPD-RNH1-HA::URA3 (pBB39)		This study
YMB11246	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1		This study
YMB11247	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1 hpr1∆::TRP1		This study
YMB11248	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3∆200 leu2∆1 hpr1∆::TRP1 CEN-HPR1::URA3 (pMB1979)		This study
YMB11251	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3-∆200 leu2∆1 hpr1∆::TRP1 CFIII (CEN3L.YPH278) HIS3 SUP11 CEN-HPR1::URA3 (pMB1979)		This study
KBY6380	MATa trp1∆63 ura3-52 his3 lys2 SPC42mCherry::NAT Ndc80-GFP::KAN		Kerry Bloom, University of North Carolina, Chapell Hill, NC
KBY6432	MATa trp1∆63 ura3-52 his3 lys2 SPC42mCherry::NAT Ndc80-GFP::KAN hpr1∆::HIS3		This study
YMB11206	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3-∆200 leu2∆1 CFIII (CEN3L.YPH278) HIS3 SUP11 URA3-Vector (pRS426)		This study
YMB11207	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3-∆200 leu2∆1 CFIII (CEN3L.YPH278) HIS3 SUP11 pGPD-RNH1-HA::URA3 (pBB39)		This study
YMB11208	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3-∆200 leu2∆1 hpr1∆::TRP1 CFIII (CEN3L.YPH278) HIS3 SUP11 URA3-Vector (pRS426)		This study
YMB11209	MATα ura3-52 lys2-801 ade2-101 trp1∆63 his3-∆200 leu2∆1 hpr1∆::TRP1 CFIII (CEN3L.YPH278) HIS3 SUP11 pGPD-RNH1-HA::URA3 (pBB39)		This study
YMB11494	MATa trp1∆63 ura3-52 his3 lys2 SPC42mCherry::NAT Ndc80-GFP::KAN URA3-Vector (pRS426)		This study
YMB11495	MATa trp1∆63 ura3-52 his3 lys2 SPC42mCherry::NAT Ndc80-GFP::KAN pGPD-RNH1-HA::URA3 (pBB39)		This study
YMB11496	MATa trp1∆63 ura3-52 his3 lys2 SPC42mCherry::NAT Ndc80-GFP::KAN hpr1∆::HIS3 URA3-Vector (pRS426)		This study
YMB11497	MATa trp1∆63 ura3-52 his3 lys2 SPC42mCherry::NAT Ndc80-GFP::KAN hpr1∆::HIS3 pGPD-RNH1-HA::URA3 (pBB39)		This study

Plasmids
Plasmid	Description		Reference
pBB39	pGPD-RNH1-HA::URA3, 2-μ		Bak et al., 2013
pRS426	URA3 Vector, 2-μ		Christianson et al., 1992
pMB1979	CEN-HPR1::URA3		Dharmacon

Primers
Locus	Forward (5′–3′)	Reverse (5′ –3′)	Reference
CEN1	CTCGATTTGCATAAGTGTGCC	GTGCTTAAGAGTTCTGTACCAC	Choy et al., 2011
CEN3	GATCAGCGCCAAACAATATGG	AACTTCCACCAGTAAACGTTTC	Paul Megee, Oakland University, Rochester, MI
CEN6	ACCTTGAAGACTATATTTCTTTT CATCACGTG	GGTCGTCCAATATCATCGTAAACGTG	John Choy, National Cancer Institute, Bethesda, MD (Basrai lab)
CEN7	AAGAGCTTCGATAAATTTGAAAATTAATTGTACTAC	CCTTGCATTATAATTATCCAA TACTTTGTCGTC	John Choy, National Cancer Institute, Bethesda, MD (Basrai lab)
CEN8	CCTTGAATGTACAGCTCTAATTACACAC	GATAATGTCTTAACCAATTTT CTAAGTTCGGAAC	John Choy, National Cancer Institute, Bethesda, MD (Basrai lab)
ACT1	ACAACGAATTGAGAGTTGCCCCAG	AATGGCGTGAGGTAGAGAGAAACC	Crotti and Basrai, 2004
169K	CATTATCAATCCTTGCGTTTCA	GCTCGAGTAATACCGGAGTGTC	Costantino and Koshland, 2018
179K	AAAGCTTTGTTTGCGGTATGTT	CCCGGATTACAAAGTCACTACC	Costantino and Koshland, 2018
310K	TCTCGGAATTTATCATGACCCAT	AAACCCTGCACACATTTCGT	Laloraya et al., 2000
ADR1	AAGCCAGATAGCGGCAACT	CACTCACAGCTGGCATTAACA	Ciftci-Yilmaz et al., 2018
GAL83	TTCATCGTCATCATCGTCGT	AAGGTCGTTTCCTGCACAAT	Ciftci-Yilmaz et al., 2018
CSE4	CCATTTGCAAGGCTAGTGAAAG	CCAATAATCCTACCAGATACGC	Sultan Ciftci-Yilmaz, Lars Boeckmann, National Cancer Institute, Bethesda, MD (Basrai lab)
SCM3	ATGAAAACCAATAAGAAAATTTCTAAAAG	TCCTTTTTGGTCTTCCGTTTTTCG	This study
TUB2	ATTGACGGCAATTGGCTCT	ACCAGTGCAAGAAAGCTTTTC	Carole Carter, National Cancer Institute, Bethesda, MD (Basrai lab)
SLD2	AGCTGAAAACATGGGAGCAT	TCGCCTTACCGTAAACTTGG	Ciftci-Yilmaz et al., 2018
RRP12	CATTTCTTCTGGATTGGCTGA	ACATTGAGAGGCAGCTTGGT	Ciftci-Yilmaz et al., 2018