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. 2021 Mar 1;32(5):402–412. doi: 10.1091/mbc.E20-02-0092

FIGURE 1:

FIGURE 1:

Depletion of ILK and culture on soft substrata synergistically promote TGFβ1-induced apoptosis in NMuMG mouse mammary epithelial cells. (A) Phase-contrast images of shcntl and shILK cells cultured on soft (E∼130 Pa) or stiff (E∼4020 Pa) fibronectin-coated polyacrylamide substrata. Scale bars, 50 μm. (B) Immunoblotting analysis for ILK normalized to GAPDH in lysates from shcntl and shILK cells. (C) Phase-contrast images of shcntl and shILK cells cultured on soft or stiff substrata and treated with TGFβ1 (2 ng/ml). Scale bars, 100 μm. (D) Quantification of caspase-3 activity in shcntl and shILK cells cultured on soft or stiff substrata and treated with or without TGFβ1 for 24 h. Error bars represent SEM of three (B) or six (D) independent replicates; ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.