FIGURE 2:

Depletion of ILK prevents TGFβ1-induced EMT in NMuMG mouse mammary epithelial cells. (A) Immuno­blotting analysis for E-cadherin, αSMA, Snail, vimentin, or GAPDH in shcntl and shILK cells cultured on soft or stiff substrata and treated with or without TGFβ1 for 24 h; immunoblotting replicates are shown in Supplemental Figure S1. (B) Quantification of immunoblotting analysis; shown are mean ± SD of three independent replicates. Color coding indicates relative increase (red) or decrease (blue) in expression, compared with shcntl cells on soft substrata in the absence of TGFβ1. (C) Immunofluorescence analysis for E-cadherin (green), Snail (magenta), and nuclei (blue) in shcntl and shILK cells cultured on soft or stiff substrata and treated with or without TGFβ1 for 24 h. Scale bars, 10 μm.