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. 2021 Mar 1;32(5):402–412. doi: 10.1091/mbc.E20-02-0092

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© 2021 Kilinc et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — Depletion of ILK strengthens cell–cell adhesion. Immunofluorescence analysis for F-actin (green), nuclei (blue), and (A) α-catenin (αcat; magenta) or (B) β-catenin (βcat; magenta) in shcntl and shILK cells. Scale bars, 20 μm. (C) Immunoblotting analysis for E-cadherin (Ecad), αcat, and βcat in shcntl and shILK cells. (D) Immunoblotting analysis for Triton X-100-insoluble or soluble fractions of Ecad, αcat, and βcat in lysates from shcntl and shILK cells. Quantification shows mean ± SD for three independent experiments; immunoblotting replicates and statistical analysis are shown in Supplemental Figure S5.