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. 2021 Apr 23;17(4):e1009554. doi: 10.1371/journal.ppat.1009554

Fig 7. Non palmitoylated GP5 co-localizes with M in a perinuclear region.

Fig 7

BHK-21 cells were transfected with GP5-HA wt and M-His wt (upper panel), or with non-acylated GP5-HA SSS and M-His SS (second from above), or with GP5-HA wt and non-acylated M-His SS (second from below), or with non-acylated GP5-HA SSS and M-His wt (lower panel). GP5 was stained with polyclonal antibodies against the HA-tag followed by secondary anti-rabbit antibodies coupled to Alexa Fluor-568 fluor, M with monoclonal anti-M antibodies followed by secondary anti-mice antibodies coupled to Alexa Fluor-488 and nuclei with DAPI. Right panel: Co-localization of M with GP5 from at least 40 cells was quantified with the Pearson’s correlation coefficient method using the JACoP plugin of the ImageJ software. Around 80% of M and GP5 pixels overlapped regardless of whether they are palmitoylated or not.