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. 2021 May 5;16(5):e0250202. doi: 10.1371/journal.pone.0250202

Fig 4. Detection of SARS-CoV-2 in clinical nasopharyngeal samples.

Fig 4

NP patient samples in VTM were tested using the LAMP Direct Assay (A,B,C) or the RNA precipitation Assay following the HMS Assay (D,E) to detect SARS-CoV-2 using the (A,D) NEB Gene N-A, (B,E) HMS Assay 1e and the (C) NEB orf1a-A oligonucleotides. Positive and negative samples are paired with Table 1 (Direct Assay) and Table 2 (RNA precipitation Assay). (F) Two by two table showing positive and negative samples detected by the Direct Assay and the RNA precipitation Assay. Each dot represents an individual experiment. Critical value threshold was set at 2 (red line). Samples 1 to 29 are true positives by RT-qPCR. Samples from 30 to 59 are true negatives by RT-qPCR.