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. 2021 Apr 21;12:663487. doi: 10.3389/fgene.2021.663487

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Copyright © 2021 Chen, Abu Zaid, Reiter, Czader, Wang, McGuire, Xuei, Gao, Huang, Abonour, Walker and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular composition was consistent between fresh and frozen/thawed CD138⁺ cells. (A) UMAP plot of 37,463 fresh and 49,203 frozen CD138⁺ cells from 6 MM patient samples, where each cell is grouped into one of 22 clusters. Each cluster is distinguished by a different color and clusters are numbered from 0 to 21. (B) UMAP plots of cells identified in fresh and frozen samples from each patient. Cluster colors and numbers are the same as in (A). (C) Scatter plot of the percentage of cells in each cluster in fresh samples (x-axis) vs. frozen samples (y-axis). Each dot represents one of 22 clusters; the color indicates a different patient.