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. 2021 Apr 29;184(9):2454–2470.e26. doi: 10.1016/j.cell.2021.03.023

Figure S2.

Figure S2

Immune profiling of NPE and NPE-IE tumors reveals recapitulation of human disease and dynamic immune populations, related to Figure 2

(A) Survival analysis of BL6 mice orthotopically injected with NPE (left), NPE-BL6-TD (center) or NPE-IE (right) cells and subjected to IP injection of anti-CD8 or isotype matched control (IgG2b). NPE: n = 6 mice + ɑCD8, n = 5 mice + ɑIgG2b; p = 0.1002. NPE-BL6-TD: n = 6 mice + ɑCD8, n = 4 mice + ɑIgG2b; p = 0.1705. NPE-IE: n = 12 + ɑCD8, n = 10 + ɑIgG2b; p = 0.0171).

(B) Example gating strategy to determine immune cell populations in normal and tumor-burdened whole brains.

(C) Quantification of the proportions of major immune cell populations in normal whole brains and tumor-burdened brains.

(D) Quantification of PD1+ TIM3+ CD4 and CD8 T cells in whole brains of non-transplanted BL6 mice versus those with tumors from transplanted NPE or NPE-IE cells; n = 4 brains analyzed for each condition.

(E) Representative images of cell classification training used for macrophage quantification in NPE/NPE-IE fluorescent IHC images.

(F) Quantification of Iba1+ F4/80+ macrophage populations as fraction of the total cell population (right). p values calculated with one-way ANOVA, n = 3 – 5 brains from each condition analyzed.