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. 2021 Apr 27;10:e65108. doi: 10.7554/eLife.65108

Figure 2. Lack of adiponectin (APN) in aging mice worsens glucose and lipid homeostasis.

(A) Body weights during aging processes for wild-type (WT) and adiponectin null (APN-KO) mice fed on chow diet. (B) Body weights during aging processes for WT and APN-KO mice fed on high-fat diet (HFD). (C) An oral glucose tolerance test (OGTT) (2 g kg−1 body weight; single gavage) on chow diet-feeding WT and APN-KO mice at 110 weeks old (n = 7 per group). (D) An OGTT (1.25 g kg−1 body weight; single gavage) on HFD-feeding WT and APN-KO mice at 85-weeks old (n = 8 for WT, n = 7 for APN-KO mice). (E) Triglyceride (TG) clearance test (20% intralipid; 15 μl g−1 body weight; single gavage) in chow diet-feeding WT and APN-KO mice at 110 weeks old (n = 9 for WT, n = 10 for APN-KO mice). (F) TG clearance test (20% intralipid; 15 μl g−1 body weight; single gavage) in HFD-feeding WT and APN-KO mice at 85 weeks old (n = 8 per group). (G) Metabolic cage analyses showing food intake for chow diet-feeding WT in APN-KO mice at 110 weeks old (n = 8 for WT, n = 7 for APN-KO mice). Data are mean ± SEM. Student’s t test: *p<0.05, **p<0.01, ***p<0.001 for WT vs. APN-KO. (H) Metabolic cage analyses showing respiratory exchange rate (RER) chow diet-feeding WT and APN-KO mice at 110 weeks old (n = 8 for WT, n = 7 for APN-KO mice). Data are mean ± SEM. Student’s t test: *p<0.05, **p<0.01, ***p<0.001 for WT vs. APN-KO.

Figure 2.

Figure 2—figure supplement 1. Body composition of wild-type (WT) mice and adiponectin null (APN-KO) mice.

Figure 2—figure supplement 1.

(A) Fat mass, lean mass, and relative subcutaneous, visceral, and brown fat pad weights of 140-weeks old WT(n=5) and APN-KO (n=5) mice fed chow diet or 100-week-old WT (n = 5–7) and APN-KO mice (n = 5) fed on high-fat diet (HFD) (B) Bone mineral content, bone mineral density, spinal bone mineral density, and femoral bone mineral density of 140-week-old WT and APN-KO mice fed on chow diet (n = 5 per group). (C) Bone mineral content, bone mineral density, spinal bone mineral density, and femoral bone mineral density of 100-week-old WT (n = 7) and APN-KO mice (n = 4) fed HFD. (D) No difference in insulin levels during oral glucose tolerance tests (OGTTs) in aged WT and APN-KO mice on HFD (n = 8 for WT, n = 7 for APN-KO mice). (E) The relative wet kidney weight with respect to body weight of 140-week-old WT and APN-KO mice fed on chow diet (n = 5 for WT, n = 6 for APN-KO mice). Bar, 100 μm. Data are mean ± SEM. Student’s t test: *p<0.05, **p<0.01, ***p<0.001 for WT vs. APN-KO.