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. 2021 Apr 27;10:e65108. doi: 10.7554/eLife.65108

Figure 3. Deletion of adiponectin (APN) in aged mice exacerbates functional decline.

Figure 3.

(A) H&E staining of an Epi fat depot of 20-week-old and 100-week-old wild-type (WT) and adiponectin null (APN-KO) mice fed on high-fat diet (HFD) or 140-week-old WT and APN-KO mice on chow diet. (B) Mac2 staining of an Epi fat depot of 20-week-old and 100-week-old WT and APN-KO mice fed on HFD or 140-week-old WT and APN-KO mice on chow diet. (C) Trichrome staining of kidney sections reveals severe interstitial and periglomerular fibrosis in 110-week-old APN-KO mice fed on HFD and 140-week-old APN-KO mice fed on chow diet. Collapsed tufts are seen inside widened Bowman’s capsules forming glomerular cysts (red arrow). (D) Mac2 staining of kidney sections of 20-week-old and 100-week-old WT and APN-KO mice fed on HFD or chow diet. (E) H&E staining of liver of 20-week-old and 100-week-old WT and APN-KO mice fed on HFD, 140-week-old WT and APN-KO mice on chow diet. Note the extensive inflammatory cell infiltrates in the liver of the aged APN-KO mice fed on HFD. (F) Trichrome and Picrosirius stains of liver sections from 100-week-old WT and APN-KO mice fed on HFD or 140-week-old WT and APN-KO mice on chow diet examine liver fibrosis. Bar, 100 μm. Data are mean ± SEM. Student’s t test: *p<0.05, **p<0.01, ***p<0.001 for WT vs. APN-KO.