Skip to main content
. 2021 Apr 27;10:e65108. doi: 10.7554/eLife.65108

Figure 4. Absence of adiponectin (APN) in aged mice exacerbates inflammation and accelerates aging.

(A) Expression of inflammatory markers in epididymal fat depots of 140-week-old wild-type (WT) and adiponectin null (APN-KO) mice fed on chow diet and 100-week-old WT and APN-KO fed on HFD(n = 10 per group). (B) Expression of inflammatory markers in kidneys of 140-week-old WT and APN-KO mice fed on chow diet and 100-week-old WT and APN-KO HFD (n = 8–10 per group). (C) FACS analysis of percentages of total macrophages, Kupffer cells, and monocytes-derived macrophages isolated from 100-week-old WT and APN-KO mice fed on HFD (n = 3 per group). (D) Expression of inflammatory and fibrosis markers in liver tissues of 20-week-old and 100-week-old WT and APN-KO mice fed on HFD (n = 5 per group of young cohorts, n = 6 per group of aged cohorts). (E) Serum AST and ALT activities in 100-week-old WT and APN-KO mice fed on HFD (n = 6 per group). (F) β-Galactosidase staining of kidney and liver sections from 100-week-old WT and APN-KO mice fed on HFD or 140-week-old WT and APN-KO mice on chow diet examines cellular senescence. (G) Expression of senescence biomarkers in kidneys and livers of 140-week-old WT (n = 6 or 10) and APN-KO mice fed on chow diet (n = 10). (H) Expression of senescence biomarkers in kidneys and livers of 100-week-old WT (n = 7–10) and APN-KO mice fed on HFD (n = 8–10). Bar, 100 μm. Data are mean ± SEM. Student’s t test: *p<0.05, **p<0.01, ***p<0.001 for WT vs. APN-KO.

Figure 4.

Figure 4—figure supplement 1. Adiponectin deficiency in aged mice exacerbates inflammation and accelerates aging.

Figure 4—figure supplement 1.

(A) Expression of inflammatory and fibrosis markers in liver of 140-week-old wild-type (WT) and adiponectin null (APN-KO) mice fed on chow diet (n = 7 for WT, n = 7 for APN-KO mice). (B) Serum corticosterone level in 100-week-old WT and APN-KO mice fed on HFD or 140-week-old WT and APN-KO mice fed on chow diet (n = 5 per group). (C) IL1β, TNFα, CD11b, F4/80, CD206, Chil3 mRNA expression were measured from FACS-sorted hepatocytes and macrophages isolated from 100-week-old WT and APN-KO mice fed on HFD (n = 6 per group). Bar, 100 μm. Data are mean ± SEM. Student’s t test: *p<0.05, **p<0.01, ***p<0.001 for WT vs. APN-KO.