Table 3. Phenotypes in Cables2d Epi KO and Cables2d Epi rescue chimeras.
|
Tetraploid embryo + Cables2d/d; ROSA26YFP/+ ESC
(Epi KO chimera) |
Wild-type chimera | |||||
|---|---|---|---|---|---|---|
| Embryonic days (E) | Total number of embryos | Phenotype | Total number of embryos | Phenotype | ||
| Normal | Abnormal | Normal | Abnormal | |||
| E6.5 | 2 | 1 | 1 | 4 | 3 | 1 |
| E7.5 | 15 | 4 | 11 | 14 | 11 | 3 |
| E8.5 | 13 | 0 | 13* | 7 | 6 | 1 |
| Tetraploid embryo + Cables2d/d; ROSA26YFP/+; CAG-tdTomato-2A-Cables2-3xFLAG ESC (Epi rescue chimera) |
Wild-type chimera | |||||
| Embryonic days (E) | Total number of embryos | Phenotype | Total number of embryos | Phenotype | ||
| Normal | Abnormal | Normal | Abnormal | |||
| E7.5 | 5 | 4 | 1 | 2 | 1 | 1 |
| E8.5 | 4 | 3 | 1 | 5 | 4 | 1 |
| E9.5 | 2 | 2 | 0 | 3 | 2 | 1 |
* All embryos had A-P axis specification.