Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (human) | CACNA1S | Wu et al., 2016 | Q13698 | |
| Gene (rabbit) | CACNA1S | Grabner et al., 1998 | P07293 | |
| Cell line (mouse) | GLT, dysgenic skeletal myotubes | Powell et al., 1996 | GLT; mdg/mdg | CaV1.1-null |
| Transfected construct (rabbit) | GFP-CaV1.1e (wild type) |
Tuluc et al., 2009 | CaV1.1- D exon 29 |
|
| Transfected construct (rabbit) | GFP-CaV1.1e -E87A/E90A, -E87A, -E90A |
This paper | ||
| Antibody | Rabbit polyclonal anti-GFP | Invitrogen Thermo Fisher |
A-6455, RRID:AB_221570 | IF (1:10,000) |
| Antibody | Mouse monoclonal anti-RyR | Invitrogen Thermo Fisher |
(MA3-925) 34 C RRID: AB_2254138 |
IF (1:500) |
| Software, algorithm | AMBER simulation software | AmberMD | RRID: SCR_014230 | |
| Software, algorithm | AmberTools 19 | AmberMD | RRID: SCR_018497 | |
| Software, algorithm | PyMOL | Schrödinger | RRID: SCR_000305 | |
| Software, algorithm | Clampex | Clampex | Version 10.2 RRID:SCR_011323 | |
| Software, algorithm | Clampfit | Clampfit | Version 10.7 RRID:SCR_011323 | |
| Software, algorithm | SigmaPlot | SigmaPlot | Version 12.0 RRID:SCR_003210 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism | Version 7 RRID:SCR_002798 |