Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | BL21(DE3) | Agilent Technologies | Agilent Technologies: 200131 | |
| Recombinant DNA reagent | pnEA-vH encoding His6-DSep1 (plasmid) | PMID:24633326 | ||
| Recombinant DNA reagent | pnCS encoding DSep2 and Pnut-StrepII (plasmid) | PMID:24633326 | ||
| Recombinant DNA reagent | pnCS encoding mEGFP-DSep2 and Pnut-StrepII (plasmid) | PMID:27473911 | ||
| Recombinant DNA reagent | pnEA-vH encoding His6-DSep1ΔC56 (plasmid) | this paper | See Materials and methods section, Septin purification | |
| Recombinant DNA reagent | pnCS encoding DSep2ΔC111 and PnutΔC123-StrepII (plasmid) | this paper | See Materials and methods section, Septin purification | |
| Recombinant DNA reagent | pnEA-vH encoding mouse SEPT2 (plasmid) | PMID:24633326 | ||
| Recombinant DNA reagent | pnCS encoding human SEPT6 and human SEPT7-StrepII (plasmid) | PMID:24633326 | ||
| Peptide, recombinant protein | Drosophila melanogaster septin hexamers composed of His6-DSep1-, DSep2, Pnut-StrepII | PMID:24633326 | ||
| Peptide, recombinant protein | Coiled-coil truncation mutant of Drosophila melanogaster septin hexamers composed of His6-DSep1ΔC56, DSep2ΔC111and PnutΔC123-StrepII | this paper | See Materials and methods, Section Septin purification | |
| Peptide, recombinant protein | Drosophila melanogaster septin hexamers composed of His6-DSep1, mEGFP-DSep2, and Pnut-StrepII | PMID:27473911 | ||
| Peptide, recombinant protein | Mammalian septin hexamers composed of mouse His6-SEPT2, human SEPT6, and human SEPT7-StrepII | PMID:24633326 | ||
| Peptide, recombinant protein |
Budding yeast septin octamers composed of Cdc3, Cdc10, Cdc11, and His6-Cdc12 | PMID:18550837 | ||
| Commercial assay, kit | HisTrap FF crude column | GE Healthcare | 17-5286-01 | |
| Commercial assay, kit | StrepTrap HP | GE Healthcare | 28-9075-46 | |
| Commercial assay, kit | 4–15% gradient Mini-PROTEAN TGX Precast Protein Gels | Bio-Rad | #4561084 | |
| Commercial assay, kit | Precision Plus Protein Kaleidoscope protein standard | Bio-Rad | 1610375 | |
| Biological Sample (genus Tobamovirus) | Tobacco mosaic virus (TMV) rods |
Godon et al., 2017
PMID:28017791 |
Gift from Dr. J.L. Pellequer (Institut de Biologie Structurale, Grenoble, France) |