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Culturing of JHH-273 in CRC conditions. (A) Phase contrast image of JHH-273 CRC cells. (B) The IDH1 mutation was present in cultured cells. (C) Induction of senescence in FBS evidenced by acidic β-galactosidase staining of JHH-273 (arrows), as well as increased p21 expression in (D). CRC, conditionally reprogrammed cells; FBS, fetal bovine serum; IDH, isocitrate dehydrogenase.
