Figure 1.

TRAF6 shows high expression in fatty liver caused by HFD

(A) The statistical plot for body weight, liver weight, and liver index in chow-fed mice and HFD-fed mice. (B) The scatterplot for FPG, FINS, and HOMA-IR in chow-fed mice and HFD-fed mice. (C) H&E staining for liver tissues in chow-fed mice and HFD-fed mice (400×). (D) Oil red O staining for fatty liver tissues in chow-fed mice and HFD-fed mice (200×). (E) The scatterplot for the contents of TG and TC in liver tissues of chow-fed and HFD-fed mice. (F) Histogram representing TNF-α levels in serum of chow-fed mice and HFD-fed mice. (G) Western blot analysis of CPT1 protein expression in liver tissues of chow-fed and HFD-fed mice and its statistical diagram. (H) Filipin staining for the fatty liver cell model (200×) and the quantitative histogram. (I) Nile red staining for the fatty liver cell model (200×) and the statistical histogram. (J) Histogram of FFA content in liver cells after OA/PA induction. (K) The histogram for the content of TG and TC in the fatty liver cell model. (L) The expression of TRAF6 in fatty liver tissues of chow-fed and HFD-fed mice as detected by immunohistochemistry (400×). (M) qRT-PCR determination of TRAF6 expression in fatty liver tissues of chow-fed mice and HFD-fed mice and the statistical histogram. (N) The histogram for TRAF6 expression in the fatty liver cell model as detected by qRT-PCR. (O) The protein expression of TRAF6 in the fatty liver cell model as detected by western blot analysis. These data were measurement data, expressed as mean ± standard deviation. Data between two groups were compared utilizing independent sample t test, while data among multiple groups at variant time points were analyzed through repeated-measures ANOVA, followed by Bonferroni post hoc test. Compared with chow or control, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. In chow-fed mice and HFD-fed mice, each n = 10. In hepatocytes used as control and induced with OA/PA, each n = 3.