Figure 6.

miR-429 targets and downregulates PPARα expression

(A) The targeted binding site between miR-429 and PPARα mRNA 3′ UTR. (B) PPARα expression in HFD-fed mice and chow-fed mice as detected by immunohistochemistry. (C) PPARα expression after induction with OA/PA as detected by qRT-PCR. (D) PPARα expression after induction with OA/PA as detected by western blot analysis. (E) The efficiency of miR-429 oe as detected by qRT-PCR. (F) The targeting relationship between miR-429 and PPARα as verified by dual luciferase reporter gene assay. (G) The overexpressing and silencing efficiency of miR-429 as detected by qRT-PCR. (H) The expression of PPARα after different treatment as detected by qRT-PCR. (I) The expression of PPARα after different treatment as detected by western blot analysis. These data were measurement data, expressed as mean ± standard deviation. Data among multiple groups were compared with the use of one-way ANOVA, followed by a Tukey’s test. ∗∗p < 0.01, ∗∗∗p < 0.001 versus control or mimic-NC. ##p < 0.01, ###p < 0.001 versus inhibitor-NC. In tissue experiment, n = 10. The cell experiment was repeated three times.