Figure 9.

TRAF6 promotes the occurrence of fatty liver through the EZH2/miR-429/PPARα axis

(A) The expression of TRAF6, EZH2, and PPARα after different treatment as detected by western blot analysis. (B) The expression of miR-429 after different treatment as detected by qRT-PCR. (C) The statistical plot for body weight, liver weight, and liver index in mice with different treatment. (D) H&E staining for liver tissues in mice with different treatment (200×). (E) Oil red O staining for fatty liver tissues in mice with different treatment (200×). (F) The scatterplot for the content of TG and TC in liver tissues of chow-fed mice and HFD-fed mice. These data were measurement data, expressed as mean ± standard deviation. Data among multiple groups were compared with the use of one-way ANOVA, followed by a Tukey’s test. ∗p < 0.05 versus chow-fed mice. ###p < 0.001 versus sh-NC. $$$p < 0.001 versus sh-TRAF6 + sh-NC. n = 10.