Table 2.

Box-Behnken experimental design runs. Results are given as mean ± SD (n = 3).

Independent variables				Dependent variables
No.	Tween 80 conc. (A) (mg/ml)	Lecithin conc. (B) (mg)	GMS conc. (C) (mg)	Size (Y1) (nm)	ZP (Y2) (mV)	EE (Y3) (%)
1	1.25	100	100	147.90 ± 16.96	−21.2 ± 2.21	75.02 ± 0.99
2	0.50	200	500	96.15 ± 3.37	−26.46 ± 1.41	87.5 ± 1.65
3	0.50	300	300	116.66 ± 2.91	−32.13 ± 3.43	71.39 ± 5.53
4	0.50	200	100	142.43 ± 3.49	−33.16 ± 3.23	72.53 ± 3.66
5	1.25	200	300	146.46 ± 6.44	−27.56 ± 2.87	51.40 ± 6.70
6	2.00	200	100	115.76 ± 3.02	−32.26 ± 3.56	36.17 ± 3.76
7	1.25	300	500	215.10 ± 13.38	−28.30 ± 3.37	47.16 ± 5.80
8	1.25	100	500	131.60 ± 10.87	−24.16 ± 0.81	84.86 ± 1.23
9	0.50	100	300	223.03 ± 12.98	−23.96 ± 0.85	84.73 ± 3.04
10	1.25	200	300	197.23 ± 14.57	−26.70 ± 1.38	77.11 ± 2.43
11	2.00	300	300	181.53 ± 10.95	−31.36 ± 3.76	71.56 ± 1.25
12	2.00	100	300	164.33 ± 27.81	−24.03 ± 3.43	78.4 ± 1.73
13	2.00	200	500	264.36 ± 12.37	−26.13 ± 0.20	76.13 ± 1.44
14	1.25	300	100	188.43 ± 12.71	−35.8 ± 1.32	71.53 ± 3.74
15	1.25	200	300	202.30 ± 8.14	−26.7 ± 0.60	86.10 ± 10