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. 2021 May 6;92:107486. doi: 10.1016/j.compbiolchem.2021.107486

Table 1.

Algorithms or rules for the rational design of siRNA molecules.

No. Rules Name Rules
1. Ui-Tei rules
  • 1

    A/U at the 5′ terminus of the sense strand

  • 2

    G/C at the 5′ terminus of the antisense strand

  • 3

    At least 4 A/U residues in the 5′ terminal 7bp of the sense strand

  • 4

    No GC stretch longer than 9nt
2. Amarzguioui rules
  • 1

    Duplex End A/U differential > 0.

  • 2

    Strong binding of 5′ sense strand

  • 3

    No U at position 1.

  • 4

    Presence of A at position 6.

  • 5

    Weak binding of 3′ sense strand.
3. Reynolds rules
  • 1

    GC content 30 %–52 % (1 point)

  • 2

    Occurrence of 3 or more

  • 3

    A/U base pair at position 15−19 of the sense strand (Each A/U base pair in this region earns 1 point)

  • 4

    Low internal stability at the target site (melting temperature Tm>-20°c) (1 point)

  • 5

    Presence of A at position 19 of the sense strand (1 point)

  • 6

    Presence of A at position 3 of the sense strand (1 point)

  • 7

    Presence of U at position 10 of the sense strand (1 point)

  • 8

    Absence of G at position 13 of the sense strand (1 point)

  • 9

    Threshold for efficient siRNAs score> = 6