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HA oxidation reduces its binding to CD44-Fc. HA-coated plates were treated with CAP to induce oxidation of HA molecules. A significant reduction in the binding levels was observed when compared to the untreated (UT) control. Wells coated only with 2% BSA (treated and untreated) served as negative controls. Results were analyzed using the Dunnett post hoc test. Mean ± S.D. * = p ≤ 0.01; *** = p ≤ 0.001; **** = p ≤ 0.0001.
