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. 2021 Apr 11;43:101968. doi: 10.1016/j.redox.2021.101968

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Oxidation of CD44⁺cells affects their binding to HA and availability at the cell surface.(A) The binding of fluorescein-HA to CD44⁺ cells treated with CAP was analyzed by flow cytometry. The results show a decrease in binding immediately after CAP treatment in all cell lines. (B) Representative histograms of each treatment condition per cell line. (C) CAP treatment increased the ΔMFI of CD44 PE in U87-MG and A375 cells, but not in HT29. (D) Representative histograms of each treatment condition per cell line. A and B: mean ΔMFI ±S.D. * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001; **** = p ≤ 0.0001 when compared to their corresponding sham-treated (ST) control. Results were analyzed using the Dunnett post hoc test.